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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
15/11/2017 |
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Data da última atualização: |
23/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOMES, S. V. F.; PORTUGAL, L. A.; ANJOS, J. P. dos; JESUS, O. N. de; OLIVEIRA, E. J. de; DAVID, J. P.; DAVID, J. M. |
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Afiliação: |
SILVANA V. F. GOMES, UFBA; LINDOMAR A. PORTUGAL, UFBA; JEANCARLO P. DOS ANJOS, UFBA; ONILDO NUNES DE JESUS, CNPMF; EDER JORGE DE OLIVEIRA, CNPMF; JUCENI P. DAVID, UFBA; JORGE M. DAVID, UFBA. |
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Título: |
Accelerated solvent extraction of phenolic compounds exploiting a Box-Behnken design and quantification of five flavonoids by HPLC-DAD in Passiflora species. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Microchemical Journal, V.132, P.28-35, 2017. |
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ISSN: |
0026-265X |
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Idioma: |
Inglês |
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Conteúdo: |
This work describes the development of a method for the extraction of phenolic compounds, mainly flavonoids, from species of Passiflora by employing accelerated solvent extraction (ASE) and using a Box-Behnken design with desirability functions for optimization. The optimal extraction conditions consisted of an extraction temperature of 80 °C, 64% (w/w) ethanol and five number of extraction cycles. A high-performance liquid chromatography-diode array detection (HPLC-DAD) method for the identification and quantification of orientin, isoorientin, vitexin, isovitexin and rutin flavonoids in the leaves of seventeen Passiflora spp. using gradient elution with acetonitrile (solvent B) and 0.2% (w/w) formic acid in water (solvent A) as the eluent mixture was also developed and validated. These five flavonoids were quantified with good linearity, LOQs, LODs, precision, and accuracy. Higher concentrations of isoorientin, orientin, vitexin, isovitexin and rutin were determinate in P. edulis f. flavicarpa (1.61 mg/g extract and 0.58 mg/g dry plant), P. morifolia (2.10 mg/g extract and 0.90 mg/g dry plant), P. setacea (2.48 mg/g extract and 0.97 mg/g dry plant), P. setacea (8.46 mg/g extract and 3.30 mg/g dry plant) and P. galbana (3.48 mg/g extract and 1.02 mg/g dry plant), respectively. |
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Thesagro: |
Maracujá. |
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Thesaurus Nal: |
Passiflora. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02017naa a2200229 a 4500
001 2079765
005 2017-11-23
008 2017 bl uuuu u00u1 u #d
022 $a0026-265X
100 1 $aGOMES, S. V. F.
245 $aAccelerated solvent extraction of phenolic compounds exploiting a Box-Behnken design and quantification of five flavonoids by HPLC-DAD in Passiflora species.$h[electronic resource]
260 $c2017
520 $aThis work describes the development of a method for the extraction of phenolic compounds, mainly flavonoids, from species of Passiflora by employing accelerated solvent extraction (ASE) and using a Box-Behnken design with desirability functions for optimization. The optimal extraction conditions consisted of an extraction temperature of 80 °C, 64% (w/w) ethanol and five number of extraction cycles. A high-performance liquid chromatography-diode array detection (HPLC-DAD) method for the identification and quantification of orientin, isoorientin, vitexin, isovitexin and rutin flavonoids in the leaves of seventeen Passiflora spp. using gradient elution with acetonitrile (solvent B) and 0.2% (w/w) formic acid in water (solvent A) as the eluent mixture was also developed and validated. These five flavonoids were quantified with good linearity, LOQs, LODs, precision, and accuracy. Higher concentrations of isoorientin, orientin, vitexin, isovitexin and rutin were determinate in P. edulis f. flavicarpa (1.61 mg/g extract and 0.58 mg/g dry plant), P. morifolia (2.10 mg/g extract and 0.90 mg/g dry plant), P. setacea (2.48 mg/g extract and 0.97 mg/g dry plant), P. setacea (8.46 mg/g extract and 3.30 mg/g dry plant) and P. galbana (3.48 mg/g extract and 1.02 mg/g dry plant), respectively.
650 $aPassiflora
650 $aMaracujá
700 1 $aPORTUGAL, L. A.
700 1 $aANJOS, J. P. dos
700 1 $aJESUS, O. N. de
700 1 $aOLIVEIRA, E. J. de
700 1 $aDAVID, J. P.
700 1 $aDAVID, J. M.
773 $tMicrochemical Journal, V.132, P.28-35, 2017.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
19/03/2012 |
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Data da última atualização: |
07/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BORTOLETI, K. C. de A.; ISEPPON, A. M. B.; MELO, N. F. de; VIDAL, A. C. B. |
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Afiliação: |
KYRIA CILENE DE A. BORTOLETI, UNIVASF; ANA MARIA BENKO-ISEPPON, UFPE; NATONIEL FRANKLIN DE MELO, CPATSA; ANA CHRISTINA BRASILEIRO VIDAL, UFPE. |
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Título: |
Chromatin differentiation between Vigna radiata (L.) R. Wilczek and V. unguiculata (L.) Walp. (Fabaceae). |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Plant Systematics and Evolution, v. 298, p. 689-693, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The present work reassesses the karyotype of both species also using CMA 3 /DAPI fluorochrome staining, as well as FISH with 5S and 45S rDNA probes, revealing some divergent features when compared with previously published data. Since these basic features provide impor- tant information for further construction of cytological maps, their reassessment is relevant for future marker associations, being here revisited and discussed. |
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Palavras-Chave: |
CMA3/DAPI; Feijão caupi; Heterocromatina; RDNA. |
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Thesagro: |
Feijão; Vigna Unguiculata. |
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Thesaurus NAL: |
Vigna radiata. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/55945/1/Natoniel-2012.pdf
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Marc: |
LEADER 01145naa a2200241 a 4500
001 1919299
005 2023-06-07
008 2012 bl uuuu u00u1 u #d
100 1 $aBORTOLETI, K. C. de A.
245 $aChromatin differentiation between Vigna radiata (L.) R. Wilczek and V. unguiculata (L.) Walp. (Fabaceae).$h[electronic resource]
260 $c2012
520 $aThe present work reassesses the karyotype of both species also using CMA 3 /DAPI fluorochrome staining, as well as FISH with 5S and 45S rDNA probes, revealing some divergent features when compared with previously published data. Since these basic features provide impor- tant information for further construction of cytological maps, their reassessment is relevant for future marker associations, being here revisited and discussed.
650 $aVigna radiata
650 $aFeijão
650 $aVigna Unguiculata
653 $aCMA3/DAPI
653 $aFeijão caupi
653 $aHeterocromatina
653 $aRDNA
700 1 $aISEPPON, A. M. B.
700 1 $aMELO, N. F. de
700 1 $aVIDAL, A. C. B.
773 $tPlant Systematics and Evolution$gv. 298, p. 689-693, 2012.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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